Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24481 | 3' | -55.9 | NC_005264.1 | + | 63426 | 0.66 | 0.926456 |
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Target: 5'- -----cCGCCCGAU-GGCGCUUugcGUGCc -3' miRNA: 3'- cucagaGCGGGCUAcCCGUGAA---CACG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 3448 | 0.66 | 0.909377 |
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Target: 5'- gGGGUCcgcCGCCCGu--GGCGCccgGUGCc -3' miRNA: 3'- -CUCAGa--GCGGGCuacCCGUGaa-CACG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 86143 | 0.66 | 0.90321 |
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Target: 5'- uGAGcCUCGUCaCGGUGGGCGacagccugGUGg -3' miRNA: 3'- -CUCaGAGCGG-GCUACCCGUgaa-----CACg -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 23940 | 0.67 | 0.890182 |
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Target: 5'- uGGUCgCGCcgCCGAUGGGUAUgUGggGCg -3' miRNA: 3'- cUCAGaGCG--GGCUACCCGUGaACa-CG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 116953 | 0.68 | 0.82093 |
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Target: 5'- uGAG-C-CGCCCGAUGGuGuCGCUUGcauugGCg -3' miRNA: 3'- -CUCaGaGCGGGCUACC-C-GUGAACa----CG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 72851 | 0.69 | 0.766773 |
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Target: 5'- -cGUCgCGCUCGAUcGGCAUaggUGUGCa -3' miRNA: 3'- cuCAGaGCGGGCUAcCCGUGa--ACACG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 103013 | 0.7 | 0.70432 |
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Target: 5'- uGAG-CUCGCuuGGUGcGGCGCUaccggcaagcaugGUGCg -3' miRNA: 3'- -CUCaGAGCGggCUAC-CCGUGAa------------CACG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 58062 | 0.71 | 0.64754 |
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Target: 5'- gGAGaCUCGCgUGGUGGGCAUUUcugGCg -3' miRNA: 3'- -CUCaGAGCGgGCUACCCGUGAAca-CG- -5' |
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| 24481 | 3' | -55.9 | NC_005264.1 | + | 48928 | 1.12 | 0.001841 |
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Target: 5'- aGAGUCUCGCCCGAUGGGCACUUGUGCg -3' miRNA: 3'- -CUCAGAGCGGGCUACCCGUGAACACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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