Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24484 | 5' | -55.4 | NC_005264.1 | + | 137619 | 0.7 | 0.757485 |
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Target: 5'- uCUGCCGGCACUgcccuggcCAcCGuguGcGCCAACAc -3' miRNA: 3'- -GACGGCCGUGAa-------GUaGCu--C-CGGUUGU- -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 144660 | 0.67 | 0.897825 |
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Target: 5'- -aGCCGGCAUUcuugCGcCGAGcgucaGCCGACAa -3' miRNA: 3'- gaCGGCCGUGAa---GUaGCUC-----CGGUUGU- -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 157325 | 0.7 | 0.767054 |
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Target: 5'- -cGCCgcGGCugU-CAUC-AGGCCAACAa -3' miRNA: 3'- gaCGG--CCGugAaGUAGcUCCGGUUGU- -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 159313 | 0.66 | 0.932704 |
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Target: 5'- -cGCCGcgucGCGgUUCGaCGAGGCgGACGg -3' miRNA: 3'- gaCGGC----CGUgAAGUaGCUCCGgUUGU- -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 161302 | 0.71 | 0.677682 |
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Target: 5'- uUGCCGGCGCggUCAacgccgCGGGGCCccaaaaAACGg -3' miRNA: 3'- gACGGCCGUGa-AGUa-----GCUCCGG------UUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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