Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24484 | 5' | -55.4 | NC_005264.1 | + | 20236 | 0.67 | 0.891178 |
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Target: 5'- -aGcCCGGCGuggcCaUCGUCGcGGCCGGCAu -3' miRNA: 3'- gaC-GGCCGU----GaAGUAGCuCCGGUUGU- -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 114471 | 0.67 | 0.8905 |
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Target: 5'- -cGCCucgacgcGGCACUgcgCAaCGAGGCCGcCAu -3' miRNA: 3'- gaCGG-------CCGUGAa--GUaGCUCCGGUuGU- -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 72319 | 0.67 | 0.8836 |
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Target: 5'- -cGuuGGCGguuuggcCUUCGUCGAuGGCCGAg- -3' miRNA: 3'- gaCggCCGU-------GAAGUAGCU-CCGGUUgu -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 99584 | 0.67 | 0.877916 |
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Target: 5'- -aGCCGGUGCggauucuggagggccUCAUCGAaaccggGGCCGACc -3' miRNA: 3'- gaCGGCCGUGa--------------AGUAGCU------CCGGUUGu -5' |
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| 24484 | 5' | -55.4 | NC_005264.1 | + | 40286 | 0.66 | 0.932704 |
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Target: 5'- -cGCCGcgucGCGgUUCGaCGAGGCgGACGg -3' miRNA: 3'- gaCGGC----CGUgAAGUaGCUCCGgUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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