miRNA: 3'- -CUGUAa---CGUUagaaUGUUAC-CGCGGU- -5'
miRNA: 3'- -CUGUaa---CGUUAGAau--GUUACCGCGGu -5'
miRNA: 3'- -CUGua-ACGuuAGaa-------UGUUACCGCGGU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24485 | 5' | -47.2 | NC_005264.1 | + | 131222 | 0.66 | 0.99955 |
|
Target: 5'- aGGCAUggucGCAGg---GCGAUGcGCGCCGc -3' miRNA: 3'- -CUGUAa---CGUUagaaUGUUAC-CGCGGU- -5' |
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| 24485 | 5' | -47.2 | NC_005264.1 | + | 135011 | 0.66 | 0.999782 |
|
Target: 5'- cGACAguggcGCGAUCUaggcCAAUGGCGUUc -3' miRNA: 3'- -CUGUaa---CGUUAGAau--GUUACCGCGGu -5' |
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| 24485 | 5' | -47.2 | NC_005264.1 | + | 144660 | 0.69 | 0.996072 |
|
Target: 5'- gGACuagUGUuuUCgcccccacgACAGUGGCGCCGa -3' miRNA: 3'- -CUGua-ACGuuAGaa-------UGUUACCGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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