Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 245 | 3' | -56.5 | AC_000008.1 | + | 10686 | 0.66 | 0.552676 |
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Target: 5'- gGCCGUCCGcCGUGaUCCaugCGGUUAc-- -3' miRNA: 3'- gCGGCGGGCaGUAC-AGG---GCCAAUacc -5' |
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| 245 | 3' | -56.5 | AC_000008.1 | + | 16373 | 0.66 | 0.541845 |
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Target: 5'- gGCCGCUCGUCGccugGaCCUGGg---GGg -3' miRNA: 3'- gCGGCGGGCAGUa---CaGGGCCaauaCC- -5' |
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| 245 | 3' | -56.5 | AC_000008.1 | + | 17673 | 0.67 | 0.509808 |
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Target: 5'- uGCCGCCUc-CGUuUCCCGGUgccgGGa -3' miRNA: 3'- gCGGCGGGcaGUAcAGGGCCAaua-CC- -5' |
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| 245 | 3' | -56.5 | AC_000008.1 | + | 17792 | 0.67 | 0.468395 |
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Target: 5'- uGCCGCCCGUCAggccgUGg-CCGGccAUGc -3' miRNA: 3'- gCGGCGGGCAGU-----ACagGGCCaaUACc -5' |
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| 245 | 3' | -56.5 | AC_000008.1 | + | 16304 | 0.69 | 0.400397 |
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Target: 5'- gGCCGCCCGUCGg---CCGGUg---- -3' miRNA: 3'- gCGGCGGGCAGUacagGGCCAauacc -5' |
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| 245 | 3' | -56.5 | AC_000008.1 | + | 9541 | 1.12 | 0.000333 |
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Target: 5'- aCGCCGCCCGUCAUGUCCCGGUUAUGGg -3' miRNA: 3'- -GCGGCGGGCAGUACAGGGCCAAUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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