Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24521 | 5' | -53.6 | NC_005264.1 | + | 29909 | 0.66 | 0.969436 |
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Target: 5'- cGGCGcuacCCCGUCGGcGCUAggGAGGUa -3' miRNA: 3'- cCCGCca--GGGCAGCU-CGAUa-UUCUAg -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 90830 | 0.66 | 0.967608 |
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Target: 5'- uGGCGG-CCgcgcaCGUCGAGCaagaaacagcucgcGUAGGAUCg -3' miRNA: 3'- cCCGCCaGG-----GCAGCUCGa-------------UAUUCUAG- -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 146831 | 0.67 | 0.951795 |
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Target: 5'- uGGGCGGUCUCG--GAGCauacGUGGcGGUCa -3' miRNA: 3'- -CCCGCCAGGGCagCUCGa---UAUU-CUAG- -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 44888 | 0.68 | 0.933531 |
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Target: 5'- aGGCGGgCCCGUCGucgucuggGGCgggaggaGAGAUCc -3' miRNA: 3'- cCCGCCaGGGCAGC--------UCGaua----UUCUAG- -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 20298 | 0.69 | 0.892312 |
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Target: 5'- -uGCGGcCCCGagGGGCU--GAGGUCg -3' miRNA: 3'- ccCGCCaGGGCagCUCGAuaUUCUAG- -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 56419 | 0.7 | 0.855921 |
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Target: 5'- -cGCGG-CgCCGUCGAGCUuc-GGGUCu -3' miRNA: 3'- ccCGCCaG-GGCAGCUCGAuauUCUAG- -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 136692 | 0.7 | 0.848004 |
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Target: 5'- gGGuGUGGUCCCGUUGGGCUc------- -3' miRNA: 3'- -CC-CGCCAGGGCAGCUCGAuauucuag -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 142977 | 0.7 | 0.848004 |
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Target: 5'- cGGCGGUCUC-UCGGGC-AUGAGGc- -3' miRNA: 3'- cCCGCCAGGGcAGCUCGaUAUUCUag -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 131408 | 1.12 | 0.003552 |
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Target: 5'- aGGGCGGUCCCGUCGAGCUAUAAGAUCu -3' miRNA: 3'- -CCCGCCAGGGCAGCUCGAUAUUCUAG- -5' |
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| 24521 | 5' | -53.6 | NC_005264.1 | + | 12381 | 1.12 | 0.003552 |
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Target: 5'- aGGGCGGUCCCGUCGAGCUAUAAGAUCu -3' miRNA: 3'- -CCCGCCAGGGCAGCUCGAUAUUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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