Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24529 | 5' | -55.7 | NC_005264.1 | + | 29220 | 0.67 | 0.883325 |
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Target: 5'- gUCACCgAUgggGAGCC-AGCGgucGAGGAg -3' miRNA: 3'- gAGUGGgUG---CUCGGaUCGCaa-CUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 120043 | 0.66 | 0.90321 |
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Target: 5'- --aGCUCAgGgGGUCUAGCGggGGGGAa -3' miRNA: 3'- gagUGGGUgC-UCGGAUCGCaaCUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 122663 | 0.66 | 0.909377 |
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Target: 5'- -aCACCUACuuuGGCCUGGCGacaGGGAa -3' miRNA: 3'- gaGUGGGUGc--UCGGAUCGCaacUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 35530 | 0.66 | 0.915308 |
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Target: 5'- gUCGCCCACGGGCgccuugUUGGCGUuccUGAa-- -3' miRNA: 3'- gAGUGGGUGCUCG------GAUCGCA---ACUccu -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 119862 | 0.66 | 0.924303 |
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Target: 5'- --uGCCUcgACGAGCUUAGCGUcacugccaaccagGGGGAc -3' miRNA: 3'- gagUGGG--UGCUCGGAUCGCAa------------CUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 9042 | 1.09 | 0.003209 |
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Target: 5'- aCUCACCCACGAGCCUAGCGUUGAGGAc -3' miRNA: 3'- -GAGUGGGUGCUCGGAUCGCAACUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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