Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24529 | 5' | -55.7 | NC_005264.1 | + | 51779 | 0.68 | 0.853726 |
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Target: 5'- aUCGCCCggcGCGAaCUUGGCGUUGAugucguagGGAa -3' miRNA: 3'- gAGUGGG---UGCUcGGAUCGCAACU--------CCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 123762 | 0.68 | 0.861442 |
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Target: 5'- --uGCCCGcCGAGCC-GGCGUccGGGGGu -3' miRNA: 3'- gagUGGGU-GCUCGGaUCGCAa-CUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 144904 | 0.66 | 0.90321 |
|
Target: 5'- -aCugUCGCGAGCCUggucuuucuaAGCGUgauucuagGGGGGc -3' miRNA: 3'- gaGugGGUGCUCGGA----------UCGCAa-------CUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 162166 | 0.66 | 0.90321 |
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Target: 5'- -aCACCuaagCACGGGUggAGCGggGAGGGg -3' miRNA: 3'- gaGUGG----GUGCUCGgaUCGCaaCUCCU- -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 154557 | 0.66 | 0.915308 |
|
Target: 5'- gUCGCCCACGGGCgccuugUUGGCGUuccUGAa-- -3' miRNA: 3'- gAGUGGGUGCUCG------GAUCGCA---ACUccu -5' |
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| 24529 | 5' | -55.7 | NC_005264.1 | + | 128069 | 1.09 | 0.003209 |
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Target: 5'- aCUCACCCACGAGCCUAGCGUUGAGGAc -3' miRNA: 3'- -GAGUGGGUGCUCGGAUCGCAACUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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