Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24544 | 3' | -47.2 | NC_005264.1 | + | 110376 | 0.67 | 0.999635 |
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Target: 5'- -----cGGUAgcguUUUCUGCUACGCGCa- -3' miRNA: 3'- cucucaCUAU----AAAGACGAUGUGCGcu -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 159727 | 0.68 | 0.999115 |
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Target: 5'- cGAGAaaccGAgac--CUGCUGCGCGCGAu -3' miRNA: 3'- -CUCUca--CUauaaaGACGAUGUGCGCU- -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 111553 | 0.68 | 0.999097 |
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Target: 5'- cGAGGGUGccuuc-CUGCUcgugguaACGCGCGAc -3' miRNA: 3'- -CUCUCACuauaaaGACGA-------UGUGCGCU- -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 15234 | 0.69 | 0.995402 |
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Target: 5'- aGAGgacacAGUGGUcgUUCUGCaUGCGCcuGCGAc -3' miRNA: 3'- -CUC-----UCACUAuaAAGACG-AUGUG--CGCU- -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 123816 | 0.69 | 0.995328 |
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Target: 5'- -cGAGUcaccgccGGUAcUUUCUGUUugGCGCGAa -3' miRNA: 3'- cuCUCA-------CUAU-AAAGACGAugUGCGCU- -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 35593 | 0.7 | 0.994619 |
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Target: 5'- cGGGAGUGAUAUUcguugcgcgUCU-CUGC-CGCGGc -3' miRNA: 3'- -CUCUCACUAUAA---------AGAcGAUGuGCGCU- -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 119754 | 0.96 | 0.123443 |
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Target: 5'- aGAGAGU-AUAUUUCUGCUACACGCGAg -3' miRNA: 3'- -CUCUCAcUAUAAAGACGAUGUGCGCU- -5' |
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| 24544 | 3' | -47.2 | NC_005264.1 | + | 727 | 1.11 | 0.016365 |
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Target: 5'- aGAGAGUGAUAUUUCUGCUACACGCGAg -3' miRNA: 3'- -CUCUCACUAUAAAGACGAUGUGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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