Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24550 | 3' | -51.4 | NC_005264.1 | + | 153359 | 0.66 | 0.991464 |
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Target: 5'- gUCUAGcgaggccuUGGggGAGGcGGgcucGCGCCUCGa -3' miRNA: 3'- -AGGUC--------AUCaaCUCCaUCa---UGCGGAGC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 128694 | 0.66 | 0.991464 |
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Target: 5'- gUCUAGcgaggccuUGGggGAGGcGGgcucGCGCCUCGa -3' miRNA: 3'- -AGGUC--------AUCaaCUCCaUCa---UGCGGAGC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 70473 | 0.66 | 0.990241 |
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Target: 5'- gCCAGUG--UGGGGUAGUGC-CCagGg -3' miRNA: 3'- aGGUCAUcaACUCCAUCAUGcGGagC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 150488 | 0.67 | 0.985732 |
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Target: 5'- gCCGGUAGgagcgGGGGgcGUuCGCCgCGa -3' miRNA: 3'- aGGUCAUCaa---CUCCauCAuGCGGaGC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 131565 | 0.67 | 0.985732 |
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Target: 5'- gCCGGUAGgagcgGGGGgcGUuCGCCgCGa -3' miRNA: 3'- aGGUCAUCaa---CUCCauCAuGCGGaGC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 91879 | 0.67 | 0.983921 |
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Target: 5'- gUCgaGGUAGc-GGGGUGGUugGCCgCGg -3' miRNA: 3'- -AGg-UCAUCaaCUCCAUCAugCGGaGC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 52216 | 0.68 | 0.966038 |
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Target: 5'- -gCGGUGGcgcGAGcUGGUACGCCUUGa -3' miRNA: 3'- agGUCAUCaa-CUCcAUCAUGCGGAGC- -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 140911 | 0.71 | 0.915534 |
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Target: 5'- -aCGGUAGUuacgguUGGGGUAG-ACGUCUCu -3' miRNA: 3'- agGUCAUCA------ACUCCAUCaUGCGGAGc -5' |
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| 24550 | 3' | -51.4 | NC_005264.1 | + | 5888 | 1.11 | 0.006521 |
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Target: 5'- gUCCAGUAGUUGAGGUAGUACGCCUCGg -3' miRNA: 3'- -AGGUCAUCAACUCCAUCAUGCGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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