miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2457 5' -56.5 NC_001436.1 + 1249 1.1 0.000032
Target:  5'- cCAAGGCCUGGAGGAGCCUUACCACGCc -3'
miRNA:   3'- -GUUCCGGACCUCCUCGGAAUGGUGCG- -5'
2457 5' -56.5 NC_001436.1 + 2272 0.67 0.147686
Target:  5'- gCAAGGCCUGGAGGcGUucuggUUUAaagggaaCugGCu -3'
miRNA:   3'- -GUUCCGGACCUCCuCG-----GAAUg------GugCG- -5'
2457 5' -56.5 NC_001436.1 + 2278 0.66 0.167555
Target:  5'- gAAGGcCCUGGAGGcaGGCCaUaucgaacccuacACCGgGCc -3'
miRNA:   3'- gUUCC-GGACCUCC--UCGGaA------------UGGUgCG- -5'
2457 5' -56.5 NC_001436.1 + 3823 0.69 0.09398
Target:  5'- gAGGGCCUGaaaGGGGGCCUgagaggACC-UGCc -3'
miRNA:   3'- gUUCCGGACc--UCCUCGGAa-----UGGuGCG- -5'
2457 5' -56.5 NC_001436.1 + 6754 0.66 0.172885
Target:  5'- aCGGGGCCcuccagGGGGGAaagcGCC--ACCuCGCg -3'
miRNA:   3'- -GUUCCGGa-----CCUCCU----CGGaaUGGuGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.