Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24574 | 5' | -57 | NC_005264.1 | + | 112583 | 0.69 | 0.721101 |
Target: 5'- aCGUGGuCUUCGCCGuGCUucuugaagaaGGUGUCCUc -3' miRNA: 3'- -GCACUcGAAGCGGC-UGA----------CCACAGGGu -5' |
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24574 | 5' | -57 | NC_005264.1 | + | 131566 | 0.7 | 0.661429 |
Target: 5'- aCGaGAGCgUCGCCGAUgggcguggaUGGUGcgCCCAg -3' miRNA: 3'- -GCaCUCGaAGCGGCUG---------ACCACa-GGGU- -5' |
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24574 | 5' | -57 | NC_005264.1 | + | 150487 | 0.7 | 0.661429 |
Target: 5'- aCGaGAGCgUCGCCGAUgggcguggaUGGUGcgCCCAg -3' miRNA: 3'- -GCaCUCGaAGCGGCUG---------ACCACa-GGGU- -5' |
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24574 | 5' | -57 | NC_005264.1 | + | 31298 | 1.09 | 0.002725 |
Target: 5'- gCGUGAGCUUCGCCGACUGGUGUCCCAg -3' miRNA: 3'- -GCACUCGAAGCGGCUGACCACAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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