Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24575 | 5' | -53.8 | NC_005264.1 | + | 157073 | 0.72 | 0.750356 |
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Target: 5'- aCGACGg-UCGCGUGGGUCUcccagcuuuuuUCUUGCg -3' miRNA: 3'- -GCUGCgaAGCGCGUCCAGAu----------AGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 22006 | 0.72 | 0.740648 |
|
Target: 5'- aGGCGCggCGCGUAGGUUUuuugCcCGCg -3' miRNA: 3'- gCUGCGaaGCGCGUCCAGAua--GaGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 56596 | 0.72 | 0.769452 |
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Target: 5'- uGGCGCUaCGCGCGGcccaUCUgggAUCUUGCa -3' miRNA: 3'- gCUGCGAaGCGCGUCc---AGA---UAGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 98061 | 0.75 | 0.619336 |
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Target: 5'- gGGCGCUgCGCGCAGaUC-GUUUCGCg -3' miRNA: 3'- gCUGCGAaGCGCGUCcAGaUAGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 130554 | 0.78 | 0.45189 |
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Target: 5'- gCGGCGCgugugcuaCGCGCAGGUCUcgcUCUCGg -3' miRNA: 3'- -GCUGCGaa------GCGCGUCCAGAu--AGAGCg -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 32309 | 1.11 | 0.003971 |
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Target: 5'- gCGACGCUUCGCGCAGGUCUAUCUCGCc -3' miRNA: 3'- -GCUGCGAAGCGCGUCCAGAUAGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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