Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24575 | 5' | -53.8 | NC_005264.1 | + | 78558 | 0.66 | 0.972142 |
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Target: 5'- -cGCGCUUUGCGCGucgcuuuugCUGUgUCGCa -3' miRNA: 3'- gcUGCGAAGCGCGUcca------GAUAgAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 74718 | 0.66 | 0.972142 |
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Target: 5'- gGGCGCggCGCGCGaaaCUGUCUCcuGCu -3' miRNA: 3'- gCUGCGaaGCGCGUccaGAUAGAG--CG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 8631 | 0.66 | 0.972142 |
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Target: 5'- aGGCGCUUCacCGCcacGUCUacGUUUCGCg -3' miRNA: 3'- gCUGCGAAGc-GCGuc-CAGA--UAGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 88475 | 0.66 | 0.972142 |
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Target: 5'- aCGACGCcgUUGUGU-GGaUUGUCUCGUc -3' miRNA: 3'- -GCUGCGa-AGCGCGuCCaGAUAGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 9920 | 0.66 | 0.974824 |
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Target: 5'- gCGGCGCUaucUCGCGCuAGGU---UCgaaGCa -3' miRNA: 3'- -GCUGCGA---AGCGCG-UCCAgauAGag-CG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 128946 | 0.66 | 0.974824 |
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Target: 5'- gCGGCGCUaucUCGCGCuAGGU---UCgaaGCa -3' miRNA: 3'- -GCUGCGA---AGCGCG-UCCAgauAGag-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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