Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24575 | 5' | -53.8 | NC_005264.1 | + | 139944 | 0.7 | 0.848285 |
Target: 5'- cCGACGCggugcugcuaUCGUGCccgaaccagGGGUCUAUUUCGa -3' miRNA: 3'- -GCUGCGa---------AGCGCG---------UCCAGAUAGAGCg -5' |
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24575 | 5' | -53.8 | NC_005264.1 | + | 146081 | 0.67 | 0.959351 |
Target: 5'- -aGCGCgccccCGCGCGGGUCUugguggaCUaCGCa -3' miRNA: 3'- gcUGCGaa---GCGCGUCCAGAua-----GA-GCG- -5' |
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24575 | 5' | -53.8 | NC_005264.1 | + | 149662 | 0.7 | 0.871298 |
Target: 5'- cCGcGCGCagCGCGUAGGUCgucGUCgUCGUc -3' miRNA: 3'- -GC-UGCGaaGCGCGUCCAGa--UAG-AGCG- -5' |
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24575 | 5' | -53.8 | NC_005264.1 | + | 153817 | 0.66 | 0.972142 |
Target: 5'- aGGCGCgaaCGCGuCGGGaCUAacggcggCUCGCc -3' miRNA: 3'- gCUGCGaa-GCGC-GUCCaGAUa------GAGCG- -5' |
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24575 | 5' | -53.8 | NC_005264.1 | + | 157073 | 0.72 | 0.750356 |
Target: 5'- aCGACGg-UCGCGUGGGUCUcccagcuuuuuUCUUGCg -3' miRNA: 3'- -GCUGCgaAGCGCGUCCAGAu----------AGAGCG- -5' |
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24575 | 5' | -53.8 | NC_005264.1 | + | 157705 | 0.66 | 0.96926 |
Target: 5'- gCGACGCccccgUCGUaauuuccuacGUAGGUCccagCUCGCc -3' miRNA: 3'- -GCUGCGa----AGCG----------CGUCCAGaua-GAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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