Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24575 | 5' | -53.8 | NC_005264.1 | + | 8631 | 0.66 | 0.972142 |
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Target: 5'- aGGCGCUUCacCGCcacGUCUacGUUUCGCg -3' miRNA: 3'- gCUGCGAAGc-GCGuc-CAGA--UAGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 7888 | 0.67 | 0.943007 |
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Target: 5'- aGAUGCggCGuCGgAGGUCUc-CUCGCc -3' miRNA: 3'- gCUGCGaaGC-GCgUCCAGAuaGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 7143 | 0.67 | 0.95561 |
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Target: 5'- cCGACGaaacguggUCGCGgcCAGGUCcAUC-CGCg -3' miRNA: 3'- -GCUGCga------AGCGC--GUCCAGaUAGaGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 5792 | 0.69 | 0.898948 |
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Target: 5'- uGGCGCcacgucugUUCGCGCcuGuacUCUAUUUCGCg -3' miRNA: 3'- gCUGCG--------AAGCGCGucC---AGAUAGAGCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 5534 | 0.71 | 0.831949 |
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Target: 5'- gCGGCGUcUCGCGCggagcGGGUUUcUCUuCGCa -3' miRNA: 3'- -GCUGCGaAGCGCG-----UCCAGAuAGA-GCG- -5' |
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| 24575 | 5' | -53.8 | NC_005264.1 | + | 5383 | 0.66 | 0.96896 |
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Target: 5'- cCGGCGCUcgaUUGCuugccaggggccaGCAGGgcguacgcggcaUCUGUCUCGg -3' miRNA: 3'- -GCUGCGA---AGCG-------------CGUCC------------AGAUAGAGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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