miRNA: 3'- -GGAGGUGCGcGCGuaGCCGaga-AGGu -5'
miRNA: 3'- -GGAGG---UGCGCGCGUAG---CCGAGaaggu -5'
miRNA: 3'- ggaGGUGCGCGCGUa----GC-CGAGAAGgu -5'
miRNA: 3'- ggaGGUGCGcGCGUa--GCCgAGAAGGu -5'
miRNA: 3'- gGAGGUGCGC---GCGuaGCCGAgAa------GGU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24576 | 3' | -57.7 | NC_005264.1 | + | 143739 | 0.68 | 0.776113 |
|
Target: 5'- gUCUCCGCGC-CGCcgCGGUgcagUCCu -3' miRNA: 3'- -GGAGGUGCGcGCGuaGCCGaga-AGGu -5' |
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| 24576 | 3' | -57.7 | NC_005264.1 | + | 146001 | 0.67 | 0.820058 |
|
Target: 5'- gCCUCUacgGCGUGCGCGUUaugGGCUCg---- -3' miRNA: 3'- -GGAGG---UGCGCGCGUAG---CCGAGaaggu -5' |
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| 24576 | 3' | -57.7 | NC_005264.1 | + | 154136 | 0.68 | 0.776113 |
|
Target: 5'- --gCCACGCGCGCGcagagCGaGUUCUUCa- -3' miRNA: 3'- ggaGGUGCGCGCGUa----GC-CGAGAAGgu -5' |
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| 24576 | 3' | -57.7 | NC_005264.1 | + | 156975 | 0.69 | 0.7194 |
|
Target: 5'- --aCCACGCuCGCGgcgCGGgUCUUCCu -3' miRNA: 3'- ggaGGUGCGcGCGUa--GCCgAGAAGGu -5' |
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| 24576 | 3' | -57.7 | NC_005264.1 | + | 157312 | 0.68 | 0.757611 |
|
Target: 5'- gCUCCGCGCGagaCGCcgCGGCUgUcaucaggCCAa -3' miRNA: 3'- gGAGGUGCGC---GCGuaGCCGAgAa------GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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