Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24587 | 5' | -56.4 | NC_005264.1 | + | 123691 | 0.66 | 0.884199 |
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Target: 5'- aUUCGGAcGUCgGUGGCUcacagcagGCCGGAgagGCc -3' miRNA: 3'- -AAGUCU-CAGaCGCCGA--------CGGCCUa--UGa -5' |
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| 24587 | 5' | -56.4 | NC_005264.1 | + | 29331 | 0.66 | 0.87706 |
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Target: 5'- -aCAGcGccucCUGCGGCuacgacgucUGCCGGGUACg -3' miRNA: 3'- aaGUCuCa---GACGCCG---------ACGGCCUAUGa -5' |
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| 24587 | 5' | -56.4 | NC_005264.1 | + | 95428 | 0.66 | 0.869698 |
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Target: 5'- -gCAGuuuGcUCUGCGGUUGCCcGGAgaUGCg -3' miRNA: 3'- aaGUCu--C-AGACGCCGACGG-CCU--AUGa -5' |
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| 24587 | 5' | -56.4 | NC_005264.1 | + | 57980 | 0.66 | 0.862117 |
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Target: 5'- aUCGGAGUCcccgcGCGGCgGCCGug-GCUc -3' miRNA: 3'- aAGUCUCAGa----CGCCGaCGGCcuaUGA- -5' |
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| 24587 | 5' | -56.4 | NC_005264.1 | + | 156418 | 0.68 | 0.794427 |
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Target: 5'- gUUCAGuGcCUGCGGCUGgCGGcgGu- -3' miRNA: 3'- -AAGUCuCaGACGCCGACgGCCuaUga -5' |
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| 24587 | 5' | -56.4 | NC_005264.1 | + | 129505 | 0.71 | 0.624868 |
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Target: 5'- -gCAGGGUUUgGCGGC-GCCGGAgGCg -3' miRNA: 3'- aaGUCUCAGA-CGCCGaCGGCCUaUGa -5' |
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| 24587 | 5' | -56.4 | NC_005264.1 | + | 39207 | 1.04 | 0.004532 |
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Target: 5'- cUUCAGAGUCUGCGGCUGCCGGAUACUu -3' miRNA: 3'- -AAGUCUCAGACGCCGACGGCCUAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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