Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24595 | 3' | -51.5 | NC_005264.1 | + | 129550 | 0.66 | 0.991215 |
Target: 5'- gGCUUAGGcgUCGCCG-CGugG-UCGUg -3' miRNA: 3'- -CGGAUCUa-AGUGGCaGCugCuAGCGu -5' |
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24595 | 3' | -51.5 | NC_005264.1 | + | 55446 | 0.66 | 0.992224 |
Target: 5'- gGCUUGGGUggucgauucguccUCAUCGUCGGCGAggGgGa -3' miRNA: 3'- -CGGAUCUA-------------AGUGGCAGCUGCUagCgU- -5' |
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24595 | 3' | -51.5 | NC_005264.1 | + | 27832 | 0.66 | 0.993331 |
Target: 5'- gGCCUAGAUcgcgcCACUGUCG-CGGcgGCAu -3' miRNA: 3'- -CGGAUCUAa----GUGGCAGCuGCUagCGU- -5' |
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24595 | 3' | -51.5 | NC_005264.1 | + | 25784 | 0.66 | 0.994223 |
Target: 5'- cGCCUGGAUcUGCUcaCGGCGGUCaGCu -3' miRNA: 3'- -CGGAUCUAaGUGGcaGCUGCUAG-CGu -5' |
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24595 | 3' | -51.5 | NC_005264.1 | + | 35470 | 0.66 | 0.995017 |
Target: 5'- cGCCUGacgcGAgagUGCCG-CGGcCGAUCGCGa -3' miRNA: 3'- -CGGAU----CUaa-GUGGCaGCU-GCUAGCGU- -5' |
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24595 | 3' | -51.5 | NC_005264.1 | + | 39106 | 0.66 | 0.995017 |
Target: 5'- cGUCUGGAccagCuGCUGUCGugGA-CGCGg -3' miRNA: 3'- -CGGAUCUaa--G-UGGCAGCugCUaGCGU- -5' |
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24595 | 3' | -51.5 | NC_005264.1 | + | 158133 | 0.66 | 0.995017 |
Target: 5'- cGUCUGGAccagCuGCUGUCGugGA-CGCGg -3' miRNA: 3'- -CGGAUCUaa--G-UGGCAGCugCUaGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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