Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24595 | 3' | -51.5 | NC_005264.1 | + | 131590 | 0.69 | 0.94908 |
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Target: 5'- cGCCUAGGUguggagggagcggCGCgCGUgGGCGcgCGCGg -3' miRNA: 3'- -CGGAUCUAa------------GUG-GCAgCUGCuaGCGU- -5' |
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| 24595 | 3' | -51.5 | NC_005264.1 | + | 65984 | 0.71 | 0.921241 |
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Target: 5'- cGCCUAGugucUCugCaGUCGACG-UCGUAg -3' miRNA: 3'- -CGGAUCua--AGugG-CAGCUGCuAGCGU- -5' |
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| 24595 | 3' | -51.5 | NC_005264.1 | + | 129189 | 0.71 | 0.915457 |
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Target: 5'- aCCUGGGUcggcuagCACCGUCGACcaaccGUCGCc -3' miRNA: 3'- cGGAUCUAa------GUGGCAGCUGc----UAGCGu -5' |
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| 24595 | 3' | -51.5 | NC_005264.1 | + | 129973 | 0.71 | 0.903154 |
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Target: 5'- uGCCUccaAGGUUCACC-UCGAacaUGAUCGUg -3' miRNA: 3'- -CGGA---UCUAAGUGGcAGCU---GCUAGCGu -5' |
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| 24595 | 3' | -51.5 | NC_005264.1 | + | 37244 | 0.72 | 0.889886 |
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Target: 5'- cCCUGGGgggcgcaUCACCGUgGACGAggCGCu -3' miRNA: 3'- cGGAUCUa------AGUGGCAgCUGCUa-GCGu -5' |
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| 24595 | 3' | -51.5 | NC_005264.1 | + | 159086 | 0.73 | 0.827869 |
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Target: 5'- aGCCUuGcgUcCGCCGUgaagcCGGCGAUCGCGa -3' miRNA: 3'- -CGGAuCuaA-GUGGCA-----GCUGCUAGCGU- -5' |
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| 24595 | 3' | -51.5 | NC_005264.1 | + | 39106 | 0.66 | 0.995017 |
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Target: 5'- cGUCUGGAccagCuGCUGUCGugGA-CGCGg -3' miRNA: 3'- -CGGAUCUaa--G-UGGCAGCugCUaGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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