Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24599 | 3' | -58.8 | NC_005264.1 | + | 148125 | 0.66 | 0.820885 |
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Target: 5'- gUCGGGucGGGGcCGUuaaaGUGCUCUGCUc -3' miRNA: 3'- gAGUCCu-CCCCaGCAc---CACGAGGUGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 155736 | 0.66 | 0.81248 |
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Target: 5'- -gCGcGGGGGGUUaUGGUGggCCGCCu -3' miRNA: 3'- gaGUcCUCCCCAGcACCACgaGGUGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 1023 | 0.66 | 0.803055 |
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Target: 5'- --gGGGcAGGGGcCGUucuguauucucgcGGUucuuGCUCCACCg -3' miRNA: 3'- gagUCC-UCCCCaGCA-------------CCA----CGAGGUGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 75373 | 0.67 | 0.795212 |
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Target: 5'- -gCAcGGuccauucGGaGUCGUGGUGCgcgCCACCg -3' miRNA: 3'- gaGU-CCuc-----CC-CAGCACCACGa--GGUGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 51830 | 0.67 | 0.786366 |
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Target: 5'- -cCAGGAGaGGG--GUGGgcGCUCCGCg -3' miRNA: 3'- gaGUCCUC-CCCagCACCa-CGAGGUGg -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 59760 | 0.67 | 0.749756 |
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Target: 5'- uCUCGGGGGGcacgaacuGGcCGUGGcUGUccacggccgugUCCGCCg -3' miRNA: 3'- -GAGUCCUCC--------CCaGCACC-ACG-----------AGGUGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 13287 | 0.72 | 0.459749 |
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Target: 5'- -cCAGcGAGgcGGGUCGUGGaGCUCC-CCg -3' miRNA: 3'- gaGUC-CUC--CCCAGCACCaCGAGGuGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 132314 | 0.72 | 0.459749 |
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Target: 5'- -cCAGcGAGgcGGGUCGUGGaGCUCC-CCg -3' miRNA: 3'- gaGUC-CUC--CCCAGCACCaCGAGGuGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 56519 | 0.74 | 0.399252 |
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Target: 5'- uCUguGGAGGGGUCGcccUGC-CCGCCg -3' miRNA: 3'- -GAguCCUCCCCAGCaccACGaGGUGG- -5' |
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| 24599 | 3' | -58.8 | NC_005264.1 | + | 51685 | 1.1 | 0.001524 |
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Target: 5'- gCUCAGGAGGGGUCGUGGUGCUCCACCc -3' miRNA: 3'- -GAGUCCUCCCCAGCACCACGAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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