Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 246 | 3' | -54.2 | AC_000008.1 | + | 9913 | 0.66 | 0.711019 |
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Target: 5'- cGCAGGcGGUAagcuccgCAuuUGGCGGGCGGgaUUGGu -3' miRNA: 3'- -CGUUC-CCAUa------GU--ACCGCCUGCU--GGCC- -5' |
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| 246 | 3' | -54.2 | AC_000008.1 | + | 33410 | 0.67 | 0.598919 |
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Target: 5'- uGCAAGGcgcuGUAuccaaagcUCAUGGCGGG-GACCa- -3' miRNA: 3'- -CGUUCC----CAU--------AGUACCGCCUgCUGGcc -5' |
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| 246 | 3' | -54.2 | AC_000008.1 | + | 32283 | 0.69 | 0.53237 |
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Target: 5'- aCGAGuuGGUGcUCAUGGCGGcgGCGGCUGc -3' miRNA: 3'- cGUUC--CCAU-AGUACCGCC--UGCUGGCc -5' |
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| 246 | 3' | -54.2 | AC_000008.1 | + | 9719 | 0.71 | 0.390312 |
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Target: 5'- cGCAA-GGUAggcugagcacCGUGGCGGGCGGCagCGGg -3' miRNA: 3'- -CGUUcCCAUa---------GUACCGCCUGCUG--GCC- -5' |
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| 246 | 3' | -54.2 | AC_000008.1 | + | 25183 | 0.84 | 0.052959 |
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Target: 5'- cGCGGGcGGUAaccgcauggaUCAcGGCGGACGGCCGGa -3' miRNA: 3'- -CGUUC-CCAU----------AGUaCCGCCUGCUGGCC- -5' |
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| 246 | 3' | -54.2 | AC_000008.1 | + | 10641 | 1.14 | 0.000403 |
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Target: 5'- cGCAAGGGUAUCAUGGCGGACGACCGGg -3' miRNA: 3'- -CGUUCCCAUAGUACCGCCUGCUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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