Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 246 | 5' | -59.2 | AC_000008.1 | + | 23561 | 0.66 | 0.409013 |
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Target: 5'- -gCCGcCCGcaCCGCCG-GGUCCGuUGCg -3' miRNA: 3'- uaGGCcGGCa-GGCGGCaCUAGGU-ACG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 1081 | 0.66 | 0.381568 |
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Target: 5'- uUCCuGGCgGggaagUgGCCGcgcUGGUCCGUGCa -3' miRNA: 3'- uAGG-CCGgCa----GgCGGC---ACUAGGUACG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 31267 | 0.66 | 0.372697 |
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Target: 5'- -cCCaGGCCG-CCGCCcGUGggCCcauUGCc -3' miRNA: 3'- uaGG-CCGGCaGGCGG-CACuaGGu--ACG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 24957 | 0.68 | 0.299322 |
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Target: 5'- aGUCCGGCCgGUCCGagacucgaaCCGggGGUCCc-GCg -3' miRNA: 3'- -UAGGCCGG-CAGGC---------GGCa-CUAGGuaCG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 12460 | 0.69 | 0.25428 |
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Target: 5'- -aCCGGCUcUCCGCaauucuggaagcgGUGGUCCcgGCg -3' miRNA: 3'- uaGGCCGGcAGGCGg------------CACUAGGuaCG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 18148 | 0.69 | 0.231479 |
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Target: 5'- -gCCGcCCGUCagGCCGUGGccggCCAUGCc -3' miRNA: 3'- uaGGCcGGCAGg-CGGCACUa---GGUACG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 25236 | 0.75 | 0.087764 |
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Target: 5'- -cCCGGUCGUCCGCCaUGAUaCCcuUGCg -3' miRNA: 3'- uaGGCCGGCAGGCGGcACUA-GGu-ACG- -5' |
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| 246 | 5' | -59.2 | AC_000008.1 | + | 10681 | 1.09 | 0.000211 |
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Target: 5'- uAUCCGGCCGUCCGCCGUGAUCCAUGCg -3' miRNA: 3'- -UAGGCCGGCAGGCGGCACUAGGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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