miRNA: 3'- -GCcUUCgCGUCCCGAGuGCUGCCaaa -5'
miRNA: 3'- gCCU--UCGCGUC-CCGAGUg----CUGCCAaa -5'
miRNA: 3'- -GCCuuCGCGUcc------CGaGUGCUGCCAAA- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24613 | 5' | -57.3 | NC_005264.1 | + | 31673 | 0.69 | 0.682981 |
|
Target: 5'- gCGcGAG-GCAGGGCUCuCGGCGGg-- -3' miRNA: 3'- -GCcUUCgCGUCCCGAGuGCUGCCaaa -5' |
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| 24613 | 5' | -57.3 | NC_005264.1 | + | 6787 | 0.68 | 0.751929 |
|
Target: 5'- gGGAugGGUGCGGcGGCUCAgagggGACGGUc- -3' miRNA: 3'- gCCU--UCGCGUC-CCGAGUg----CUGCCAaa -5' |
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| 24613 | 5' | -57.3 | NC_005264.1 | + | 66178 | 0.66 | 0.871621 |
|
Target: 5'- cCGGggGUGCGcuaacgccGCgCGCGACGGUUUu -3' miRNA: 3'- -GCCuuCGCGUcc------CGaGUGCUGCCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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