Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24616 | 5' | -49.9 | NC_005264.1 | + | 18513 | 0.66 | 0.997605 |
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Target: 5'- cUGUCGAg--CAUgGugGGGguCCUCAGu -3' miRNA: 3'- aACAGCUuuaGUAgCugCUC--GGAGUC- -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 16734 | 0.66 | 0.997153 |
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Target: 5'- aUGUUGugcgcgaUAUCGACGuGCCUCAc -3' miRNA: 3'- aACAGCuuua---GUAGCUGCuCGGAGUc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 98504 | 0.66 | 0.996035 |
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Target: 5'- cUGcCGGAggCGcCGACGgggGGCCUCAGu -3' miRNA: 3'- aACaGCUUuaGUaGCUGC---UCGGAGUC- -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 104069 | 0.66 | 0.995354 |
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Target: 5'- -gGUCGAcGAUUA-CGGCGAGCCcaUCAu -3' miRNA: 3'- aaCAGCU-UUAGUaGCUGCUCGG--AGUc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 109273 | 0.69 | 0.977386 |
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Target: 5'- -cGUCGGGcgCGUCGuaccGCGAGCUUCc- -3' miRNA: 3'- aaCAGCUUuaGUAGC----UGCUCGGAGuc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 116416 | 0.7 | 0.962235 |
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Target: 5'- -cGUCGAGcgCAgggCGGCGugcAGCCUCGc -3' miRNA: 3'- aaCAGCUUuaGUa--GCUGC---UCGGAGUc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 2488 | 0.73 | 0.856452 |
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Target: 5'- --uUCGAcGAUgGUCGGCGGGCCUCGc -3' miRNA: 3'- aacAGCU-UUAgUAGCUGCUCGGAGUc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 121515 | 0.73 | 0.856452 |
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Target: 5'- --uUCGAcGAUgGUCGGCGGGCCUCGc -3' miRNA: 3'- aacAGCU-UUAgUAGCUGCUCGGAGUc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 56220 | 0.76 | 0.725244 |
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Target: 5'- -cGUCGAGAUCAcUGGCGAGCgUCGc -3' miRNA: 3'- aaCAGCUUUAGUaGCUGCUCGgAGUc -5' |
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| 24616 | 5' | -49.9 | NC_005264.1 | + | 68903 | 1.08 | 0.012965 |
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Target: 5'- gUUGUCGAAAUCAUCGACGAGCCUCAGg -3' miRNA: 3'- -AACAGCUUUAGUAGCUGCUCGGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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