Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24620 | 3' | -48.1 | NC_005264.1 | + | 113300 | 0.66 | 0.999634 |
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Target: 5'- cGCGuACgUGGccauGUCUCGUGUcacaaguUGCAGGu -3' miRNA: 3'- uUGCuUGgACC----UAGAGUAUA-------ACGUCC- -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 137555 | 0.66 | 0.999135 |
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Target: 5'- cGCGGACCcagGGAaaaacaaguaUCUCAUGggcgUGgAGGg -3' miRNA: 3'- uUGCUUGGa--CCU----------AGAGUAUa---ACgUCC- -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 6135 | 0.68 | 0.997285 |
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Target: 5'- cGCGAAgcaccgcccguCCUGGGUCUCGc---GCGGGc -3' miRNA: 3'- uUGCUU-----------GGACCUAGAGUauaaCGUCC- -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 77774 | 0.68 | 0.996191 |
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Target: 5'- cGGCGGACCUGGGgaaCGgg--GCAGGc -3' miRNA: 3'- -UUGCUUGGACCUagaGUauaaCGUCC- -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 51788 | 0.69 | 0.99292 |
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Target: 5'- cGCGAACUUGGcgUUgAUGUcGUAGGg -3' miRNA: 3'- uUGCUUGGACCuaGAgUAUAaCGUCC- -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 79437 | 0.69 | 0.990598 |
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Target: 5'- gGGCGGugCgGGGUCUCcgGggGCAGa -3' miRNA: 3'- -UUGCUugGaCCUAGAGuaUaaCGUCc -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 116154 | 0.7 | 0.982169 |
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Target: 5'- uGGCGAACCguUGGGUCUCGaccaGUGGGg -3' miRNA: 3'- -UUGCUUGG--ACCUAGAGUauaaCGUCC- -5' |
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| 24620 | 3' | -48.1 | NC_005264.1 | + | 70251 | 1.08 | 0.018585 |
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Target: 5'- uAACGAACCUGGAUCUCAUAUUGCAGGc -3' miRNA: 3'- -UUGCUUGGACCUAGAGUAUAACGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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