Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24620 | 5' | -56.7 | NC_005264.1 | + | 159645 | 0.68 | 0.764101 |
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Target: 5'- gGUCCUGCGAaauaagcccUUCCCCGCgCCGUCg -3' miRNA: 3'- -CAGGGCGUUga-------AGGGGGUGaGGUAGa -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 14278 | 0.69 | 0.754599 |
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Target: 5'- uGUCCCacaaauaagGCAuggGCUcuaguuUCCCCCACgCCAUCa -3' miRNA: 3'- -CAGGG---------CGU---UGA------AGGGGGUGaGGUAGa -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 132404 | 0.69 | 0.74499 |
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Target: 5'- -gCCCGCGGC-UCCCCCGagacCUCC-UCc -3' miRNA: 3'- caGGGCGUUGaAGGGGGU----GAGGuAGa -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 13377 | 0.69 | 0.74499 |
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Target: 5'- -gCCCGCGGC-UCCCCCGagacCUCC-UCc -3' miRNA: 3'- caGGGCGUUGaAGGGGGU----GAGGuAGa -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 3855 | 0.69 | 0.735283 |
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Target: 5'- gGUCCUGCGccggggauuggGCUgCCCCaCGuCUCCGUCUu -3' miRNA: 3'- -CAGGGCGU-----------UGAaGGGG-GU-GAGGUAGA- -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 60954 | 0.69 | 0.735283 |
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Target: 5'- uUCgCCGCgAACUUCagCCACUCCAUUg -3' miRNA: 3'- cAG-GGCG-UUGAAGggGGUGAGGUAGa -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 48543 | 0.69 | 0.725487 |
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Target: 5'- cGUCCCGCGcg--CCCCCGucgcCUUCGUCg -3' miRNA: 3'- -CAGGGCGUugaaGGGGGU----GAGGUAGa -5' |
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| 24620 | 5' | -56.7 | NC_005264.1 | + | 70287 | 1.07 | 0.003424 |
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Target: 5'- aGUCCCGCAACUUCCCCCACUCCAUCUu -3' miRNA: 3'- -CAGGGCGUUGAAGGGGGUGAGGUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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