Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24621 | 5' | -49.7 | NC_005264.1 | + | 48396 | 0.67 | 0.994289 |
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Target: 5'- gGCGUcGGGCgGCGGccaggAGCGggUGGGGg -3' miRNA: 3'- aUGUA-CCUGaCGCUa----UCGUuaACCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 131331 | 0.67 | 0.993365 |
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Target: 5'- gAUAUgGGugUGUGA-AGCGcGUUGGGAa -3' miRNA: 3'- aUGUA-CCugACGCUaUCGU-UAACCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 13598 | 0.67 | 0.991162 |
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Target: 5'- gACG-GGACcgGCGAUcGCGGUcGGGAc -3' miRNA: 3'- aUGUaCCUGa-CGCUAuCGUUAaCCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 132625 | 0.67 | 0.991162 |
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Target: 5'- gACG-GGACcgGCGAUcGCGGUcGGGAc -3' miRNA: 3'- aUGUaCCUGa-CGCUAuCGUUAaCCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 1662 | 0.69 | 0.97049 |
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Target: 5'- gUACAUGGGCgacgGUGG-GGCAaaguAUUGGGGc -3' miRNA: 3'- -AUGUACCUGa---CGCUaUCGU----UAACCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 3849 | 0.7 | 0.960256 |
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Target: 5'- gGCAUGGGucCUGCGccGGgGAUUGGGc -3' miRNA: 3'- aUGUACCU--GACGCuaUCgUUAACCCu -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 52888 | 0.73 | 0.87433 |
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Target: 5'- aGCGagGGGCUGCGugGUGGCA--UGGGAc -3' miRNA: 3'- aUGUa-CCUGACGC--UAUCGUuaACCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 125155 | 0.82 | 0.433371 |
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Target: 5'- gGCGUGGGCUGCGAU-GCuugUGGGGc -3' miRNA: 3'- aUGUACCUGACGCUAuCGuuaACCCU- -5' |
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| 24621 | 5' | -49.7 | NC_005264.1 | + | 72106 | 1.08 | 0.012301 |
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Target: 5'- gUACAUGGACUGCGAUAGCAAUUGGGAc -3' miRNA: 3'- -AUGUACCUGACGCUAUCGUUAACCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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