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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24630 | 5' | -50.1 | NC_005264.1 | + | 44861 | 0.67 | 0.993283 |
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Target: 5'- --uGCCGUCGAgagaucugcagccUGgGGAggcgggcccgucGUCGUCUGGg -3' miRNA: 3'- cauCGGUAGCU-------------AUgUCU------------CAGCAGACC- -5' |
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| 24630 | 5' | -50.1 | NC_005264.1 | + | 67367 | 0.69 | 0.976849 |
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Target: 5'- cGUAGCCAagaCGAgucgGCGGcGUCGUCUa- -3' miRNA: 3'- -CAUCGGUa--GCUa---UGUCuCAGCAGAcc -5' |
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| 24630 | 5' | -50.1 | NC_005264.1 | + | 57008 | 0.71 | 0.954058 |
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Target: 5'- --cGUCAUCugcaGCGGAGUCGUCUGa -3' miRNA: 3'- cauCGGUAGcua-UGUCUCAGCAGACc -5' |
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| 24630 | 5' | -50.1 | NC_005264.1 | + | 117116 | 0.78 | 0.674288 |
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Target: 5'- -gAGCCGUCGAUGCGGAGUauaCGgauugugaccagCUGGg -3' miRNA: 3'- caUCGGUAGCUAUGUCUCA---GCa-----------GACC- -5' |
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| 24630 | 5' | -50.1 | NC_005264.1 | + | 80799 | 1.13 | 0.007199 |
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Target: 5'- cGUAGCCAUCGAUACAGAGUCGUCUGGg -3' miRNA: 3'- -CAUCGGUAGCUAUGUCUCAGCAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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