Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24649 | 3' | -54.7 | NC_005264.1 | + | 76792 | 0.67 | 0.92118 |
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Target: 5'- gUUACCGUCgGCGAaguuuaGCagGUUCCUGGCGg -3' miRNA: 3'- -AGUGGCAGaCGUU------UGa-CGGGGACUGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 157445 | 0.67 | 0.92118 |
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Target: 5'- aCGCC--CUGCug---GCCCCUGGCAa -3' miRNA: 3'- aGUGGcaGACGuuugaCGGGGACUGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 59543 | 0.66 | 0.926697 |
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Target: 5'- aCGCgGUCU-CuGACUGCCCggGACAg -3' miRNA: 3'- aGUGgCAGAcGuUUGACGGGgaCUGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 73439 | 0.66 | 0.926697 |
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Target: 5'- -uGCUGUUUcgcGCAGuCUGCCCUUGGCc -3' miRNA: 3'- agUGGCAGA---CGUUuGACGGGGACUGu -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 99788 | 0.66 | 0.941779 |
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Target: 5'- uUC-UUGUUUGgGAACgcGCCCCUGGCGu -3' miRNA: 3'- -AGuGGCAGACgUUUGa-CGGGGACUGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 132896 | 0.66 | 0.94632 |
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Target: 5'- -uGCUGUgaGCGAagGCUGCCCUgcguuaaugGACAa -3' miRNA: 3'- agUGGCAgaCGUU--UGACGGGGa--------CUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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