Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24649 | 3' | -54.7 | NC_005264.1 | + | 125710 | 0.67 | 0.92118 |
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Target: 5'- cCGCCGgc-GCcuGgUGCCCCUGugGa -3' miRNA: 3'- aGUGGCagaCGuuUgACGGGGACugU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 132896 | 0.66 | 0.94632 |
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Target: 5'- -uGCUGUgaGCGAagGCUGCCCUgcguuaaugGACAa -3' miRNA: 3'- agUGGCAgaCGUU--UGACGGGGa--------CUGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 133706 | 0.67 | 0.915419 |
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Target: 5'- cCGCCGcgcaCUGguG-CUGCCUCUGGCc -3' miRNA: 3'- aGUGGCa---GACguUuGACGGGGACUGu -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 154824 | 0.71 | 0.73536 |
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Target: 5'- gCGCCGUCgugGCGGGC-GCCUCUGcCGg -3' miRNA: 3'- aGUGGCAGa--CGUUUGaCGGGGACuGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 157445 | 0.67 | 0.92118 |
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Target: 5'- aCGCC--CUGCug---GCCCCUGGCAa -3' miRNA: 3'- aGUGGcaGACGuuugaCGGGGACUGU- -5' |
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| 24649 | 3' | -54.7 | NC_005264.1 | + | 160198 | 0.74 | 0.550011 |
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Target: 5'- -uGCCG-CUGCAGAgcgcaacCUGCCCgCUGACAc -3' miRNA: 3'- agUGGCaGACGUUU-------GACGGG-GACUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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