Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24649 | 3' | -54.7 | NC_005264.1 | + | 38418 | 0.67 | 0.92118 |
Target: 5'- aCGCC--CUGCug---GCCCCUGGCAa -3' miRNA: 3'- aGUGGcaGACGuuugaCGGGGACUGU- -5' |
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24649 | 3' | -54.7 | NC_005264.1 | + | 35798 | 0.71 | 0.73536 |
Target: 5'- gCGCCGUCgugGCGGGC-GCCUCUGcCGg -3' miRNA: 3'- aGUGGCAGa--CGUUUGaCGGGGACuGU- -5' |
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24649 | 3' | -54.7 | NC_005264.1 | + | 33946 | 0.69 | 0.831652 |
Target: 5'- cCGCCGUCgucgaggacgagcggUgccGCAAGCUGUCCgCUGGCGa -3' miRNA: 3'- aGUGGCAG---------------A---CGUUUGACGGG-GACUGU- -5' |
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24649 | 3' | -54.7 | NC_005264.1 | + | 20283 | 0.72 | 0.643624 |
Target: 5'- -gACCGUCUGCGcGCUugcgGCCCCgaggGGCu -3' miRNA: 3'- agUGGCAGACGUuUGA----CGGGGa---CUGu -5' |
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24649 | 3' | -54.7 | NC_005264.1 | + | 4441 | 0.68 | 0.889961 |
Target: 5'- gUCAUCGUCUGag----GCCUCUGGCu -3' miRNA: 3'- -AGUGGCAGACguuugaCGGGGACUGu -5' |
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24649 | 3' | -54.7 | NC_005264.1 | + | 4323 | 0.72 | 0.684875 |
Target: 5'- cCACCacagcGCAGGcCUGCCCCUGGCGg -3' miRNA: 3'- aGUGGcaga-CGUUU-GACGGGGACUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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