Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24653 | 3' | -55.5 | NC_005264.1 | + | 93288 | 1.07 | 0.003793 |
Target: 5'- uCAGUGGAGGAAUGCCUCAAGGCGCUGu -3' miRNA: 3'- -GUCACCUCCUUACGGAGUUCCGCGAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 139489 | 0.7 | 0.744238 |
Target: 5'- --cUGGAGaGAAUcGCCUCGGGGCagaccccGCUGg -3' miRNA: 3'- gucACCUC-CUUA-CGGAGUUCCG-------CGAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 53070 | 0.69 | 0.773203 |
Target: 5'- aGGUGGccgcggacccagcGGGAGUGUCUaagcugguggaCAAGGCGCUc -3' miRNA: 3'- gUCACC-------------UCCUUACGGA-----------GUUCCGCGAc -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 151372 | 0.69 | 0.792804 |
Target: 5'- gAGUGcuucGGGGggUGCUUCGGGGCuguuCUGg -3' miRNA: 3'- gUCAC----CUCCuuACGGAGUUCCGc---GAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 32345 | 0.69 | 0.792804 |
Target: 5'- gAGUGcuucGGGGggUGCUUCGGGGCuguuCUGg -3' miRNA: 3'- gUCAC----CUCCuuACGGAGUUCCGc---GAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 26059 | 0.68 | 0.819648 |
Target: 5'- aGGUGGuGGccguguGUGCCUagcccGGCGCUGu -3' miRNA: 3'- gUCACCuCCu-----UACGGAguu--CCGCGAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 124117 | 0.68 | 0.835852 |
Target: 5'- cCGG-GGAGGggUccucuacGCCUgGGGGCgaGCUGg -3' miRNA: 3'- -GUCaCCUCCuuA-------CGGAgUUCCG--CGAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 5090 | 0.68 | 0.835852 |
Target: 5'- cCGG-GGAGGggUccucuacGCCUgGGGGCgaGCUGg -3' miRNA: 3'- -GUCaCCUCCuuA-------CGGAgUUCCG--CGAC- -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 16379 | 0.66 | 0.896683 |
Target: 5'- uGGgggGGAgGGGAUGCC-CGuGGCGCa- -3' miRNA: 3'- gUCa--CCU-CCUUACGGaGUuCCGCGac -5' |
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24653 | 3' | -55.5 | NC_005264.1 | + | 78473 | 0.66 | 0.90253 |
Target: 5'- uGGUGGuugcucacauuauAGGAuacGCCUCGAcGGUGUUGg -3' miRNA: 3'- gUCACC-------------UCCUua-CGGAGUU-CCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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