Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24666 | 3' | -47.4 | NC_005264.1 | + | 78739 | 0.66 | 0.999924 |
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Target: 5'- gGUCUucGUUGuucUCCGUGUcaauagcCGCGAGCa -3' miRNA: 3'- -CAGAu-CAGCua-AGGUACAa------GUGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 6265 | 0.66 | 0.99987 |
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Target: 5'- aGUCUGGucUCGGUggaCAUGUuagCGcCGAGCu -3' miRNA: 3'- -CAGAUC--AGCUAag-GUACAa--GU-GCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 142190 | 0.66 | 0.999823 |
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Target: 5'- aUCUuccaccgacgCGGUUCCGUGUaaucUCGCGAGg -3' miRNA: 3'- cAGAuca-------GCUAAGGUACA----AGUGCUCg -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 74432 | 0.66 | 0.999783 |
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Target: 5'- ----uGUCGGUgacaaCCGUGUugagcuUCGCGGGCa -3' miRNA: 3'- cagauCAGCUAa----GGUACA------AGUGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 2677 | 0.67 | 0.999723 |
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Target: 5'- -cCUAGUCGggUCCGcaa--GCGGGCu -3' miRNA: 3'- caGAUCAGCuaAGGUacaagUGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 58438 | 0.68 | 0.998735 |
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Target: 5'- cUCUGcGUCGucGUUCCAgGUUguCGGGCg -3' miRNA: 3'- cAGAU-CAGC--UAAGGUaCAAguGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 59033 | 0.69 | 0.997212 |
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Target: 5'- cGUCaGGUCGGUcuccgcgcaUCCGaaccgcgaucuuguUGUUgGCGAGCa -3' miRNA: 3'- -CAGaUCAGCUA---------AGGU--------------ACAAgUGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 154013 | 0.71 | 0.98804 |
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Target: 5'- cGUUU-GUCGuGUUCCGUGc-CGCGAGCg -3' miRNA: 3'- -CAGAuCAGC-UAAGGUACaaGUGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 34986 | 0.71 | 0.98804 |
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Target: 5'- cGUUU-GUCGuGUUCCGUGc-CGCGAGCg -3' miRNA: 3'- -CAGAuCAGC-UAAGGUACaaGUGCUCG- -5' |
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| 24666 | 3' | -47.4 | NC_005264.1 | + | 102832 | 1.13 | 0.013233 |
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Target: 5'- cGUCUAGUCGAUUCCAUGUUCACGAGCc -3' miRNA: 3'- -CAGAUCAGCUAAGGUACAAGUGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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