Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24686 | 5' | -54.3 | NC_005264.1 | + | 150697 | 0.66 | 0.949416 |
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Target: 5'- -aCGCG-CGAGGCagGGCU-CUCGGCg -3' miRNA: 3'- ugGCGUaGCUUUGa-UCGAcGAGCCGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 152464 | 0.66 | 0.953571 |
|
Target: 5'- cCCGuCGUCGcccGGAC-AGCUGCUCGcGUg -3' miRNA: 3'- uGGC-GUAGC---UUUGaUCGACGAGC-CGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 153342 | 0.67 | 0.930364 |
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Target: 5'- cGCCGCGccUGAcGCUGGCUGaCUC-GCUg -3' miRNA: 3'- -UGGCGUa-GCUuUGAUCGAC-GAGcCGA- -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 153825 | 0.68 | 0.880478 |
|
Target: 5'- -aCGCGUCGGGACUaacGGCgGCUCGcCUc -3' miRNA: 3'- ugGCGUAGCUUUGA---UCGaCGAGCcGA- -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 156418 | 0.68 | 0.894396 |
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Target: 5'- cGCCGCcgCGAGaguguACguggAGgaGUUCGGCa -3' miRNA: 3'- -UGGCGuaGCUU-----UGa---UCgaCGAGCCGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 158128 | 0.66 | 0.94038 |
|
Target: 5'- aGCgGCGUCuGGACcAGCUGCUgucguggacgCGGCg -3' miRNA: 3'- -UGgCGUAGcUUUGaUCGACGA----------GCCGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 158243 | 0.68 | 0.890319 |
|
Target: 5'- cGCCGCcgCGgcGCUGGCggggagggccugcguUcccgGCUCGGCUu -3' miRNA: 3'- -UGGCGuaGCuuUGAUCG---------------A----CGAGCCGA- -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 162297 | 0.71 | 0.73085 |
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Target: 5'- cGCCGUcucuucCGGAugGCUGGgUGCUCGGCg -3' miRNA: 3'- -UGGCGua----GCUU--UGAUCgACGAGCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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