Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24686 | 5' | -54.3 | NC_005264.1 | + | 66471 | 0.69 | 0.816136 |
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Target: 5'- cACCGCcgCGGccaGACUcaaugcuuggcgGGCUGCgCGGCg -3' miRNA: 3'- -UGGCGuaGCU---UUGA------------UCGACGaGCCGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 31669 | 0.69 | 0.833418 |
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Target: 5'- aACUGCG-CGAGGCagGGCU-CUCGGCg -3' miRNA: 3'- -UGGCGUaGCUUUGa-UCGAcGAGCCGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 50262 | 0.69 | 0.841778 |
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Target: 5'- uGCCGCGgcggCGAuuCcgAGUUGCUCGuGCg -3' miRNA: 3'- -UGGCGUa---GCUuuGa-UCGACGAGC-CGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 99009 | 0.68 | 0.865644 |
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Target: 5'- gACCGCGcCGAuACUagAGCUGgUCGaGCg -3' miRNA: 3'- -UGGCGUaGCUuUGA--UCGACgAGC-CGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 153825 | 0.68 | 0.880478 |
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Target: 5'- -aCGCGUCGGGACUaacGGCgGCUCGcCUc -3' miRNA: 3'- ugGCGUAGCUUUGA---UCGaCGAGCcGA- -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 39216 | 0.68 | 0.890319 |
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Target: 5'- cGCCGCcgCGgcGCUGGCggggagggccugcguUcccgGCUCGGCUu -3' miRNA: 3'- -UGGCGuaGCuuUGAUCG---------------A----CGAGCCGA- -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 37391 | 0.68 | 0.894396 |
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Target: 5'- cGCCGCcgCGAGaguguACguggAGgaGUUCGGCa -3' miRNA: 3'- -UGGCGuaGCUU-----UGa---UCgaCGAGCCGa -5' |
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| 24686 | 5' | -54.3 | NC_005264.1 | + | 33437 | 0.66 | 0.953571 |
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Target: 5'- cCCGuCGUCGcccGGAC-AGCUGCUCGcGUg -3' miRNA: 3'- uGGC-GUAGC---UUUGaUCGACGAGC-CGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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