Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24696 | 3' | -54.7 | NC_005264.1 | + | 108470 | 0.66 | 0.94341 |
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Target: 5'- uUCCucuGCGUC-CUCGU-CCGCUUCgCUg -3' miRNA: 3'- cAGGu--CGUAGaGAGCAcGGUGAAG-GA- -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 110164 | 0.67 | 0.911457 |
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Target: 5'- -aUCGGCcacgaAUCUCUcCGUGCC-CUUCCc -3' miRNA: 3'- caGGUCG-----UAGAGA-GCACGGuGAAGGa -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 98348 | 0.68 | 0.885286 |
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Target: 5'- gGUCCAgGCGacUCUCUCG-GCgCGCUUgCCg -3' miRNA: 3'- -CAGGU-CGU--AGAGAGCaCG-GUGAA-GGa -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 24247 | 0.68 | 0.855434 |
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Target: 5'- cUCCGGCGccgaaguugUUCUUGUGCCugGCUUCCc -3' miRNA: 3'- cAGGUCGUa--------GAGAGCACGG--UGAAGGa -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 43956 | 0.74 | 0.543144 |
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Target: 5'- aGUUCAGCAUUUCUcCGUcggucGCCACUUCUUc -3' miRNA: 3'- -CAGGUCGUAGAGA-GCA-----CGGUGAAGGA- -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 15610 | 0.74 | 0.533068 |
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Target: 5'- cUCCGGCAuaUCUCUCGUGcCCACcucgagCCUu -3' miRNA: 3'- cAGGUCGU--AGAGAGCAC-GGUGaa----GGA- -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 3553 | 1.07 | 0.004579 |
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Target: 5'- cGUCCAGCAUCUCUCGUGCCACUUCCUc -3' miRNA: 3'- -CAGGUCGUAGAGAGCACGGUGAAGGA- -5' |
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| 24696 | 3' | -54.7 | NC_005264.1 | + | 122580 | 1.07 | 0.004579 |
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Target: 5'- cGUCCAGCAUCUCUCGUGCCACUUCCUc -3' miRNA: 3'- -CAGGUCGUAGAGAGCACGGUGAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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