Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 247 | 3' | -57.6 | AC_000008.1 | + | 8662 | 0.67 | 0.342312 |
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Target: 5'- -cGCgGGCAGGAGCugGuGCuGCGcGCGu -3' miRNA: 3'- guCG-CCGUUCUCGuuCuCGuCGC-CGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 8611 | 0.66 | 0.425439 |
|
Target: 5'- gGGgGGCuccggacccgccGGGAGaGGGGGCAGgGGCAc -3' miRNA: 3'- gUCgCCG------------UUCUCgUUCUCGUCgCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 8492 | 0.69 | 0.246731 |
|
Target: 5'- aCAGUGGUggGAGCgggAcuuuccugguacaccAGGGCAGCGGg- -3' miRNA: 3'- -GUCGCCGuuCUCG---U---------------UCUCGUCGCCgu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 7501 | 0.71 | 0.190711 |
|
Target: 5'- aGGCGGUAGGuGU-GGuGCAGCGGCc -3' miRNA: 3'- gUCGCCGUUCuCGuUCuCGUCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 4607 | 0.66 | 0.396405 |
|
Target: 5'- --aUGGCAAuGGGCccacGGGCGGCGGCc -3' miRNA: 3'- gucGCCGUU-CUCGuu--CUCGUCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 2161 | 0.78 | 0.0558 |
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Target: 5'- gCAGCaGCAGGAGgAAGccaGGCGGCGGCGg -3' miRNA: 3'- -GUCGcCGUUCUCgUUC---UCGUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 1676 | 0.68 | 0.309317 |
|
Target: 5'- -uGCGGUgguGGAuGUuaucAGGGCAGCGGCGc -3' miRNA: 3'- guCGCCGu--UCU-CGu---UCUCGUCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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