Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 247 | 3' | -57.6 | AC_000008.1 | + | 9399 | 0.71 | 0.185458 |
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Target: 5'- uGGCGGCGguGGGGgAGGGGggacaCGGCGGCGa -3' miRNA: 3'- gUCGCCGU--UCUCgUUCUC-----GUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 9738 | 0.74 | 0.124361 |
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Target: 5'- -cGUGGCGGGcGGCAGcGGGCGGCGGUc -3' miRNA: 3'- guCGCCGUUC-UCGUU-CUCGUCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 18364 | 0.74 | 0.110671 |
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Target: 5'- gGGCuGGCGAcGGCGAcGGCGGCGGCGg -3' miRNA: 3'- gUCG-CCGUUcUCGUUcUCGUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 15061 | 0.77 | 0.066839 |
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Target: 5'- aGGCGGCAGcaacAGCAGuGGCAGCGGCGc -3' miRNA: 3'- gUCGCCGUUc---UCGUUcUCGUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 2161 | 0.78 | 0.0558 |
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Target: 5'- gCAGCaGCAGGAGgAAGccaGGCGGCGGCGg -3' miRNA: 3'- -GUCGcCGUUCUCgUUC---UCGUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 26704 | 0.81 | 0.037619 |
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Target: 5'- gCAGCGGCAgcGGcAGCAAcAGCAGCGGCc -3' miRNA: 3'- -GUCGCCGU--UC-UCGUUcUCGUCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 11077 | 1.08 | 0.000284 |
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Target: 5'- gCAGCGGCAAGAGCAAGAGCAGCGGCAg -3' miRNA: 3'- -GUCGCCGUUCUCGUUCUCGUCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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