Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24704 | 3' | -58.2 | NC_005264.1 | + | 63277 | 0.66 | 0.798236 |
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Target: 5'- --gGCUCGAGagaGGAcaaccgGCCCCagucgGGGGAUg -3' miRNA: 3'- uuaCGAGCUCg--UCU------UGGGG-----CCCCUGu -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 10074 | 0.66 | 0.807088 |
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Target: 5'- --aGC-CGcGGCGGAAucucCCgCCGGGGGCAu -3' miRNA: 3'- uuaCGaGC-UCGUCUU----GG-GGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 142780 | 0.66 | 0.815783 |
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Target: 5'- -uUGCUCGcuacGCGGGGCCgCGuGGcGACAu -3' miRNA: 3'- uuACGAGCu---CGUCUUGGgGC-CC-CUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 63008 | 0.66 | 0.824313 |
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Target: 5'- cGUGCUUGGcGCAcuccagccuuACCCCGGGGuCGg -3' miRNA: 3'- uUACGAGCU-CGUcu--------UGGGGCCCCuGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 19259 | 0.66 | 0.824313 |
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Target: 5'- --cGUUCccuCcGGGCCCCGGGGACGg -3' miRNA: 3'- uuaCGAGcucGuCUUGGGGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 70475 | 0.66 | 0.824313 |
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Target: 5'- cAGUGUg-GGGUAGuGCCCagGGGGGCAg -3' miRNA: 3'- -UUACGagCUCGUCuUGGGg-CCCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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