Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24704 | 3' | -58.2 | NC_005264.1 | + | 5233 | 0.7 | 0.561541 |
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Target: 5'- uAAUGCgCGAGCAGGACgCCCugcugggcuacGGGGAa- -3' miRNA: 3'- -UUACGaGCUCGUCUUG-GGG-----------CCCCUgu -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 57263 | 0.7 | 0.561541 |
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Target: 5'- --cGCUCGAGCccuAGCCCgCGGGGGu- -3' miRNA: 3'- uuaCGAGCUCGuc-UUGGG-GCCCCUgu -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 95300 | 0.73 | 0.437635 |
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Target: 5'- --cGCaugCGAGcCAGGAuacauCCCCGGGGACGu -3' miRNA: 3'- uuaCGa--GCUC-GUCUU-----GGGGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 32866 | 0.73 | 0.411276 |
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Target: 5'- cAUGUUCGAGguGAACCuuGGaGGCAg -3' miRNA: 3'- uUACGAGCUCguCUUGGggCCcCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 127216 | 1.06 | 0.002771 |
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Target: 5'- gAAUGCUCGAGCAGAACCCCGGGGACAc -3' miRNA: 3'- -UUACGAGCUCGUCUUGGGGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 8189 | 1.06 | 0.002771 |
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Target: 5'- gAAUGCUCGAGCAGAACCCCGGGGACAc -3' miRNA: 3'- -UUACGAGCUCGUCUUGGGGCCCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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