Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24704 | 3' | -58.2 | NC_005264.1 | + | 3446 | 0.69 | 0.662776 |
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Target: 5'- -cUGCUCGcaaAGCgccacgAGggUCUCGGGGGCGa -3' miRNA: 3'- uuACGAGC---UCG------UCuuGGGGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 31523 | 0.69 | 0.622138 |
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Target: 5'- --gGCUCGGGCGGGgaggGCCgUUGGGGugGg -3' miRNA: 3'- uuaCGAGCUCGUCU----UGG-GGCCCCugU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 79434 | 0.7 | 0.59171 |
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Target: 5'- cGUGggCGGuGCGGggUCuCCGGGGGCAg -3' miRNA: 3'- uUACgaGCU-CGUCuuGG-GGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 57263 | 0.7 | 0.561541 |
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Target: 5'- --cGCUCGAGCccuAGCCCgCGGGGGu- -3' miRNA: 3'- uuaCGAGCUCGuc-UUGGG-GCCCCUgu -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 8189 | 1.06 | 0.002771 |
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Target: 5'- gAAUGCUCGAGCAGAACCCCGGGGACAc -3' miRNA: 3'- -UUACGAGCUCGUCUUGGGGCCCCUGU- -5' |
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| 24704 | 3' | -58.2 | NC_005264.1 | + | 19259 | 0.66 | 0.824313 |
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Target: 5'- --cGUUCccuCcGGGCCCCGGGGACGg -3' miRNA: 3'- uuaCGAGcucGuCUUGGGGCCCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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