Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24706 | 3' | -54.9 | NC_005264.1 | + | 17141 | 0.66 | 0.944465 |
Target: 5'- --gGCUUgGGCGCGCAUGGguCGGAc- -3' miRNA: 3'- uggUGAG-CUGCGCGUACUguGCCUac -5' |
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24706 | 3' | -54.9 | NC_005264.1 | + | 117275 | 0.66 | 0.948817 |
Target: 5'- --gACUCGuGCGCGCc-GACACGGGc- -3' miRNA: 3'- uggUGAGC-UGCGCGuaCUGUGCCUac -5' |
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24706 | 3' | -54.9 | NC_005264.1 | + | 91525 | 0.66 | 0.951317 |
Target: 5'- cGCgCACUCGccaaguucaugcuCGCGCAUaucgGGCGCGGcgGg -3' miRNA: 3'- -UG-GUGAGCu------------GCGCGUA----CUGUGCCuaC- -5' |
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24706 | 3' | -54.9 | NC_005264.1 | + | 131590 | 0.66 | 0.954128 |
Target: 5'- cGCCuagguguggagggagCGGCGCGCGUGggcgcGCGCGGAg- -3' miRNA: 3'- -UGGuga------------GCUGCGCGUAC-----UGUGCCUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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