miRNA: 3'- -UGGUGaGCugCG---CGUACUGUgCCUAc -5'
miRNA: 3'- -UGGUGaGCUGCGCGUaCUGUGCcuac -5'
miRNA: 3'- -UGGUGAGCUGCGCGUACUGUGCCUAC- -5'
miRNA: 3'- -UGGUGAGCUGCGCGUACUGUGCCUAC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24706 | 3' | -54.9 | NC_005264.1 | + | 90766 | 0.73 | 0.612985 |
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Target: 5'- uGCCGC-CGugGCuguGCAUGACAgGGAUu -3' miRNA: 3'- -UGGUGaGCugCG---CGUACUGUgCCUAc -5' |
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| 24706 | 3' | -54.9 | NC_005264.1 | + | 134902 | 0.74 | 0.592568 |
|
Target: 5'- gACCAC-CGACGCGCA-GACACGc--- -3' miRNA: 3'- -UGGUGaGCUGCGCGUaCUGUGCcuac -5' |
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| 24706 | 3' | -54.9 | NC_005264.1 | + | 9007 | 1.07 | 0.005489 |
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Target: 5'- uACCACUCGACGCGCAUGACACGGAUGc -3' miRNA: 3'- -UGGUGAGCUGCGCGUACUGUGCCUAC- -5' |
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| 24706 | 3' | -54.9 | NC_005264.1 | + | 128034 | 1.07 | 0.005489 |
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Target: 5'- uACCACUCGACGCGCAUGACACGGAUGc -3' miRNA: 3'- -UGGUGAGCUGCGCGUACUGUGCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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