Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24706 | 3' | -54.9 | NC_005264.1 | + | 10308 | 0.66 | 0.939878 |
Target: 5'- uACCggGCgcagCGACGCGCAcUGagcaaGCGCGGAa- -3' miRNA: 3'- -UGG--UGa---GCUGCGCGU-AC-----UGUGCCUac -5' |
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24706 | 3' | -54.9 | NC_005264.1 | + | 9007 | 1.07 | 0.005489 |
Target: 5'- uACCACUCGACGCGCAUGACACGGAUGc -3' miRNA: 3'- -UGGUGAGCUGCGCGUACUGUGCCUAC- -5' |
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24706 | 3' | -54.9 | NC_005264.1 | + | 8062 | 0.67 | 0.924689 |
Target: 5'- cCCACgucgCGGC-CGCAgucgGGCACGGGc- -3' miRNA: 3'- uGGUGa---GCUGcGCGUa---CUGUGCCUac -5' |
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24706 | 3' | -54.9 | NC_005264.1 | + | 6736 | 0.66 | 0.944465 |
Target: 5'- -gUACUCcGCG-GCGUGGCGCGGGa- -3' miRNA: 3'- ugGUGAGcUGCgCGUACUGUGCCUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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