Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24706 | 5' | -55.7 | NC_005264.1 | + | 4736 | 0.68 | 0.861442 |
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Target: 5'- --uGCCCGcCGAGCC-GGCGUccGGGGGu -3' miRNA: 3'- gagUGGGU-GCUCGGaUCGCAa-CUCCU- -5' |
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| 24706 | 5' | -55.7 | NC_005264.1 | + | 149563 | 0.69 | 0.785399 |
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Target: 5'- aCUCuCCCGCGgacAGCCccgGGCGgaGGGGGg -3' miRNA: 3'- -GAGuGGGUGC---UCGGa--UCGCaaCUCCU- -5' |
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| 24706 | 5' | -55.7 | NC_005264.1 | + | 4011 | 0.69 | 0.785399 |
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Target: 5'- cCUCGCCCACcagGGGCUUGGCGggccgaUGcaucAGGAg -3' miRNA: 3'- -GAGUGGGUG---CUCGGAUCGCa-----AC----UCCU- -5' |
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| 24706 | 5' | -55.7 | NC_005264.1 | + | 39209 | 0.7 | 0.736985 |
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Target: 5'- -gCGCCUACGccgccgcGGCgCUGGCGggGAGGGc -3' miRNA: 3'- gaGUGGGUGC-------UCG-GAUCGCaaCUCCU- -5' |
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| 24706 | 5' | -55.7 | NC_005264.1 | + | 24308 | 0.74 | 0.526223 |
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Target: 5'- -aCAUCCACGGuGCCgAGCGUUGaAGGGg -3' miRNA: 3'- gaGUGGGUGCU-CGGaUCGCAAC-UCCU- -5' |
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| 24706 | 5' | -55.7 | NC_005264.1 | + | 119862 | 0.66 | 0.924303 |
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Target: 5'- --uGCCUcgACGAGCUUAGCGUcacugccaaccagGGGGAc -3' miRNA: 3'- gagUGGG--UGCUCGGAUCGCAa------------CUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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