Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24717 | 3' | -57.2 | NC_005264.1 | + | 95441 | 0.7 | 0.70785 |
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Target: 5'- aGCCgUCGUggaauaCGcuaCCGCGCCGgcGGGCg -3' miRNA: 3'- -CGG-AGCAa-----GCa--GGCGCGGCauCUCGa -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 96302 | 0.7 | 0.668228 |
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Target: 5'- cGCCUCGg-CGUCCGCguuGCUGUAcugcgcgaaagcGAGCUc -3' miRNA: 3'- -CGGAGCaaGCAGGCG---CGGCAU------------CUCGA- -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 98996 | 0.68 | 0.77448 |
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Target: 5'- gGCCUgaCGaaaCGaCCGCGCCGauacUAGAGCUg -3' miRNA: 3'- -CGGA--GCaa-GCaGGCGCGGC----AUCUCGA- -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 113498 | 0.66 | 0.887171 |
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Target: 5'- uGCCgCGUUCcUCCGgGCCG-AGcGCg -3' miRNA: 3'- -CGGaGCAAGcAGGCgCGGCaUCuCGa -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 124021 | 0.73 | 0.519462 |
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Target: 5'- cGUCgUCGUucUCGUCCGCGCCGgggucgccgcGGAGUa -3' miRNA: 3'- -CGG-AGCA--AGCAGGCGCGGCa---------UCUCGa -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 133212 | 1.09 | 0.002805 |
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Target: 5'- gGCCUCGUUCGUCCGCGCCGUAGAGCUu -3' miRNA: 3'- -CGGAGCAAGCAGGCGCGGCAUCUCGA- -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 137307 | 0.66 | 0.873315 |
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Target: 5'- cGCCU----UGUCCGCGCCGccuuauGGGCg -3' miRNA: 3'- -CGGAgcaaGCAGGCGCGGCau----CUCGa -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 149417 | 0.7 | 0.70785 |
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Target: 5'- cGUCUCGgcggcggCgGUCgCGCGCCGgugcggAGAGCUg -3' miRNA: 3'- -CGGAGCaa-----G-CAG-GCGCGGCa-----UCUCGA- -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 152111 | 0.68 | 0.818583 |
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Target: 5'- uGCCgUCGgu-GUCCGCGCCG-GGGGaCa -3' miRNA: 3'- -CGG-AGCaagCAGGCGCGGCaUCUC-Ga -5' |
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| 24717 | 3' | -57.2 | NC_005264.1 | + | 156377 | 0.68 | 0.783578 |
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Target: 5'- cCCgaCGUUCG-CCGCGCCGccgaGGAGUUc -3' miRNA: 3'- cGGa-GCAAGCaGGCGCGGCa---UCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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