Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2472 | 5' | -47 | NC_001437.1 | + | 9046 | 0.66 | 0.721115 |
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Target: 5'- gAGAGAAGAag-CCuGGAGaguuUGGAaaAGCUa -3' miRNA: 3'- -UCUUUUCUguaGGuCCUU----ACCU--UCGG- -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 4173 | 0.66 | 0.721115 |
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Target: 5'- uGGgcGAGAacCAUCCAGuGGAUGuGAGCg -3' miRNA: 3'- -UCuuUUCU--GUAGGUC-CUUACcUUCGg -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 5829 | 0.67 | 0.669061 |
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Target: 5'- -----cGACAUCUcuGaAAUGGggGCCa -3' miRNA: 3'- ucuuuuCUGUAGGucC-UUACCuuCGG- -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 7818 | 0.68 | 0.602987 |
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Target: 5'- uGGGAGGACAUCCGGuuucGcGAGGCUc -3' miRNA: 3'- uCUUUUCUGUAGGUCcuuaC-CUUCGG- -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 10198 | 0.68 | 0.563638 |
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Target: 5'- gGGAaagcguGAGGACAUCU-GGuGUGGuAGCCu -3' miRNA: 3'- -UCU------UUUCUGUAGGuCCuUACCuUCGG- -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 1285 | 0.7 | 0.463077 |
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Target: 5'- cGGAuguGGACuuUUCGGGAagGGAAGCa -3' miRNA: 3'- -UCUuu-UCUGu-AGGUCCUuaCCUUCGg -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 1673 | 0.72 | 0.362308 |
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Target: 5'- cGAAgGGGACGUCCAGGGGagaGcGAGGUCa -3' miRNA: 3'- uCUU-UUCUGUAGGUCCUUa--C-CUUCGG- -5' |
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| 2472 | 5' | -47 | NC_001437.1 | + | 9743 | 1.14 | 0.00039 |
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Target: 5'- cAGAAAAGACAUCCAGGAAUGGAAGCCu -3' miRNA: 3'- -UCUUUUCUGUAGGUCCUUACCUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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