Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
24720 | 5' | -53.6 | NC_005264.1 | + | 72440 | 0.66 | 0.946032 |
Target: 5'- cGCGUCgaacacgUUGUaucgGCGGGCGCcGCCggUCGGa -3' miRNA: 3'- -UGUAGa------AACGa---UGUCCGCG-CGG--AGUC- -5' |
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24720 | 5' | -53.6 | NC_005264.1 | + | 126411 | 0.66 | 0.958054 |
Target: 5'- uCGUCUccgcgGcCUGCAGGUacuugggGCGCCUCAc -3' miRNA: 3'- uGUAGAaa---C-GAUGUCCG-------CGCGGAGUc -5' |
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24720 | 5' | -53.6 | NC_005264.1 | + | 14082 | 0.66 | 0.962091 |
Target: 5'- aGCAUCagag--ACAGGCGUGCCUggaCGGg -3' miRNA: 3'- -UGUAGaaacgaUGUCCGCGCGGA---GUC- -5' |
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24720 | 5' | -53.6 | NC_005264.1 | + | 21340 | 0.66 | 0.965519 |
Target: 5'- uGCAUUgc-GCUGCcguucgucccGGGC-CGCCUCGGc -3' miRNA: 3'- -UGUAGaaaCGAUG----------UCCGcGCGGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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