Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24720 | 5' | -53.6 | NC_005264.1 | + | 143342 | 0.7 | 0.825565 |
Target: 5'- uCGUCUggcaGCUggaaccucGCAGGUGCGCgUCAGc -3' miRNA: 3'- uGUAGAaa--CGA--------UGUCCGCGCGgAGUC- -5' |
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24720 | 5' | -53.6 | NC_005264.1 | + | 70142 | 0.7 | 0.79868 |
Target: 5'- aACGUgcUUGCUGCcgcGGCgGCGCCUCAc -3' miRNA: 3'- -UGUAgaAACGAUGu--CCG-CGCGGAGUc -5' |
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24720 | 5' | -53.6 | NC_005264.1 | + | 82025 | 0.74 | 0.610332 |
Target: 5'- uCGUCUUUGUcccaagggucuacgaUgcGCAGGCGCGCCUCc- -3' miRNA: 3'- uGUAGAAACG---------------A--UGUCCGCGCGGAGuc -5' |
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24720 | 5' | -53.6 | NC_005264.1 | + | 133782 | 1.08 | 0.005146 |
Target: 5'- gACAUCUUUGCUACAGGCGCGCCUCAGc -3' miRNA: 3'- -UGUAGAAACGAUGUCCGCGCGGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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